Enzymes Lab Report Example | Topics and Well Written Essays - 1250 words

Enzymes - Lab Report ExampleFollowing addition of iodine to a solution of glucose, the only color observed was red or yellow (iodine color). Therefore, the faster the blue color is lost, the faster the enzyme amylase legal action. The enzyme amylase cant hydrolyze starch if it is inactivated, so the starch-iodine complex blue color will persist. The comportment of glucose in the samples was tested using Benedicts reagent. When blue solution of Benedicts reagent is added to a glucose solution the color changes to green (at low concentrations of glucose) or reddish-orange (at higher concentrations of glucose). Starch cant react with Benedicts reagent, therefore the solution remains blue. The aim of the experiment was to look at how enzyme amylase activity is affected by pH, temperature and concentration of the enzyme. Introduction Enzymes are proteins in nature they are catalysts for biological reactions. Like all catalysts, they speed up reactions by weighed down the reactions acti vation energy without themselves being used up. Enzymes catalyze all biochemical reactions. They can be denatured in a course of ways since they are proteins in nature, Therefore they work optimally under mild conditions. At body temperature and at a neutral pH, most of them project optimum activity. Enzymes also are known to be very specific they act only on a specific substrate or peerless type of similar substrate molecules. This is because the enzyme lively site is complementary to the polarity and shape of the substrate. Only one kind of substrate will fit into the active site. (Abu et al, 2005) Methods and Materials Preparation Water johns of constant, low and high temperature were used in the experiment. A large weewee bath was set to 37C.For low temperature bath, a 250-mL beaker, was one-half filled with tap water, and some ice was added to the water to attain between 0 and 5C temperature. High temperature water bath was made by filling a 250-mL beaker to two-thirds fu ll then heated to boil. The temperature of the bath was close to 100C. 1% starch solution was used for every experiment part. unity reagent for each experiment was also used. Commercial amylase solution, clean droppers and a white spot plate were also needed in the experiment. For starch testing, few drops of starch were transferred to one well of the spot plate. One iodine reagent drop was added. Iodine and Starch was reacted to form a deep blue-black complex. For glucose testing, 1% glucose solution of 3 ml was added in a test tube. 2 ml of Benedicts solution was added and heated in a boiling water bath for 3-4 minutes. The reaction formed a red-orange solid. Experiment 1 Effect of Enzyme Concentration Five test tubes were labeled as 1-5. 4 ml of 1 % starch was placed in every first four test tubes. 4 ml of amylase solution was placed in the fifth tube. All of the tubes were placed in water bath (37C) for 5 minutes. 5 clean droppers were obtained then labeled from 1 to 5.Separate dropper were used for each mixture to avoid contamination. Tube 1 was the control and it never had any enzyme. The tubes were momentarily removed from the water bath and quickly 3 drops of the warmed amylase solution was added to tube 2, to tube 3, 6 drops of amylase was added and in tube 4, 10 drops of amylase was added. The tubes were mixed quickly by shaking gently then later put immediately back into 37C water bath. The time at which the enzyme was added was